| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 531 | Holdemania filiformis | AGACGCTGCAGGCTTTGA | TCCGTTCATCGTTCGGCA | 59.57 | 59.35 | 201 | 63 |
100.00%
|
50.00%
|
| Primer 532 | Holdemania filiformis | TGCGCTGGGTGATCGAAA | TGCTGCGCACAATCCCTT | 59.65 | 60.28 | 186 | 63 |
100.00%
|
50.00%
|
| Primer 533 | Holdemania filiformis | AGTCTTGGGGTTCTGCAGC | CCGGTTTCCTGACAACGGA | 59.93 | 59.93 | 236 | 63 |
100.00%
|
50.00%
|
| Primer 534 | Holdemania filiformis | CCGGAGCGATGATCAAGCT | TCACGCCAGTTTCGCCTT | 59.93 | 59.89 | 248 | 63 |
100.00%
|
50.00%
|
| Primer 535 | Holdemania filiformis | ACGCGATCGCTCTGTTGA | TTGCCATAACGCTTGCGC | 59.43 | 59.82 | 181 | 63 |
100.00%
|
50.00%
|
| Primer 536 | Holdemania filiformis | ACGACAACGCGACACTGT | TCGGTTCACGGTTGCCTT | 59.90 | 59.49 | 282 | 63 |
100.00%
|
50.00%
|
| Primer 537 | Holdemania filiformis | AGGCGTGCGTGGAAGATT | ATTTCAGGCTGGCTGCGA | 59.65 | 59.65 | 271 | 63 |
100.00%
|
50.00%
|
| Primer 538 | Holdemania filiformis | ACTGCAACGGACGCAAAG | TTCGCTTCTTCCCGTGTCC | 58.98 | 60.01 | 277 | 63 |
100.00%
|
50.00%
|
| Primer 539 | Holdemania filiformis | TTTACGTCGACGGCAGCT | TAACTTTCCTCCCGACGCG | 59.35 | 59.79 | 171 | 62 |
100.00%
|
50.00%
|
| Primer 540 | Holdemania filiformis | AGTTGTGCTGTGGGGATCG | CCGGATTCAACCTCGACCA | 60.00 | 59.41 | 159 | 62 |
100.00%
|
50.00%
|